206-849-9811 contact@OPT.bio

Our Services

DNA Construction and Cloning

New Design

Design and synthesis of new DNA encoding protein of interest in an expression vector

 

Construction of Expression Vector

Subcloning of existing protein-encoding DNA into an expression vector

Antibody Panels

Construction of heavy and light chain expression vectors for multiple antibodies in parallel

Hybridoma Antibody Variable Domain Sequencing

Determine nucleotide sequences of variable domains from antibody heavy and light chains derived from hybridomas

Expression in Mammalian Cells

HEK293 and CHO

Test for Transient Expression in Mammalian Cells

Small scale test for expression of new construct in mammalian cell system

Scale Up Transient Expression in Mammalian Cells

Constructs that pass the expression test are scaled up in ranges spanning culture volumes of 100 ml to tens of liters

Antibody Panels

Expression of multiple recombinant antibodies in parallel

Stable Pool Generation

Construction of pools of stable transfected cells 

Expression in Bacterial Cells

E.coli expression for soluble proteins and refolded from inclusion bodies

Test for Expression in Bacterial Cells

Small scale test for expression of new construct in bacterial cell system

Scale Up Expression in Bacterial Cells

Constructs that pass the expression test are scaled up in ranges spanning culture volumes of 100 ml to tens of liters

Protein Purification

Purification from Mammalian Cells

Purification of secreted proteins from culture supernatants

Purification from Bacterial Cells

Purification of soluble proteins from bacterial cell lysates

Protein Refolding

Protein refolding from Ecoli inclusion bodies. Optimization of refolding conditions if needed

Chromatography Polishing Steps

Additional chromatography steps to achieve further protein purify 

Antibody Panels

Purification of panels of antibodies and other protein types – various scales available

 

Protein Modifications

post-translational and in-vivo modifications of proteins

Fluorescent Labeling

Conjugation of fluorescent labels to proteins

Biotinylation

Addition of biotin either at site-specific positions (in vivo or in-vitro) or random positions (in-vitro)

Others

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 Protein Analysis

Protein Engineering

We will work with you to establish a design goal for your protein engineering project

Structure-Based Analysis

Analysis incorporating structural and bioinformatic aspects for translating design goal elements to a list of testable protein variants

Single or Multiple Point Mutants and Screening

Focused exploration of protein variants

Library Design and Screening

Broad exploration of protein variants

Epitope Mapping

Design strategies, make reagents, and execute plans for obtaining epitope map information for antibody  programs