Our Services
DNA Construction and Cloning
New Design
Design and synthesis of new DNA encoding protein of interest in an expression vector
Construction of Expression Vector
Subcloning of existing protein-encoding DNA into an expression vector
Antibody Panels
Construction of heavy and light chain expression vectors for multiple antibodies in parallel
Hybridoma Antibody Variable Domain Sequencing
Determine nucleotide sequences of variable domains from antibody heavy and light chains derived from hybridomas
Expression in Mammalian Cells
HEK293 and CHO
Test for Transient Expression in Mammalian Cells
Small scale test for expression of new construct in mammalian cell system
Scale Up Transient Expression in Mammalian Cells
Constructs that pass the expression test are scaled up in ranges spanning culture volumes of 100 ml to tens of liters
Antibody Panels
Expression of multiple recombinant antibodies in parallel
Stable Pool Generation
Construction of pools of stable transfected cells
Expression in Baculovirus-infected Insect Cells
Sf9 and Hi5
Test for Expression in Insect Cells
Small scale test for expression of new construct in insect cell system
Scale Up Expression in Insect Cells
Constructs that pass the expression test are scaled up in ranges spanning culture volumes of 100 ml to tens of liters
Expression in Bacterial Cells
E.coli expression for soluble proteins and refolded from inclusion bodies
Test for Expression in Bacterial Cells
Small scale test for expression of new construct in bacterial cell system
Scale Up Expression in Bacterial Cells
Constructs that pass the expression test are scaled up in ranges spanning culture volumes of 100 ml to tens of liters
Protein Purification
Purification from Mammalian Cells
Purification of secreted proteins from culture supernatants
Purification from Insect Cells
Purification of intracellular expressed proteins and secreted proteins from culture supernatants
Purification from Bacterial Cells
Purification of soluble proteins from bacterial cell lysates
Protein Refolding
Protein refolding from Ecoli inclusion bodies. Optimization of refolding conditions if needed
Chromatography Polishing Steps
Additional chromatography steps to achieve further protein purify
Antibody Panels
Purification of panels of antibodies and other protein types – various scales available
Protein Modifications
post-translational and in-vivo modifications of proteins
Fluorescent Labeling
Conjugation of fluorescent labels to proteins
Biotinylation
Addition of biotin either at site-specific positions (in vivo or in-vitro) or random positions (in-vitro)
Others
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Protein Analysis
Protein Engineering
We will work with you to establish a design goal for your protein engineering project
Structure-Based Analysis
Analysis incorporating structural and bioinformatic aspects for translating design goal elements to a list of testable protein variants
Single or Multiple Point Mutants and Screening
Focused exploration of protein variants
Library Design and Screening
Broad exploration of protein variants
Epitope Mapping
Design strategies, make reagents, and execute plans for obtaining epitope map information for antibody programs